First install ActivePerl for Windows. 1. install IO-stringy from the DOS command line cd IO-stringy-2.110 folder type 'perl makefile' (this creates the makefile) use nmake to install the package (nmake works just like make in unix) type 'nmake' [return] type 'nmake install' [return] 2. install most recent DB_File with ppm (perl package manager) (must be connected to internet for this) from windoows command line, type 'ppm' type 'search DB_File' type 'install DB_File ####' #### = most recent version 3. install bioperl modules with nmake just like you did with IO_Stringy After the bioperl installs, it will tell you if there are any other required external dependencies, but there shouldn't be.