Mathematical Biology seminar
Randal Dull
Department of Anesthesiology
University of Utah
"Endothelial Glycocalyx: Mechano-transduction and capillary permeability"
November 30, 2005
3:05pm, LCB 215
The endothelial glycocalyx is a cell-surface layer composed of
glycoproteins, glycolipids, glycosaminoglycans and adsorbed serum proteins
that, collectively, forms a three-dimensional polymer matrix . We have
recently demonstrated that components of the glycocalyx participate
in mechano-transduction which, in turn, influences endothelial barrier
function. To further investigate the role of the glycocalyx in endothelial
barrier function, we have developed a tissue-engineered lung capillary
model and have developed biophysical techniques to characterize the
structure-function relationship of the glycocalyx. I will provide an
overview of the techniques I use to measure lung capillary permeability,
the use of fluorescence correlation spectroscopy (FCS) to make mass
transport measurements directly inside the glycocalyx and the use of
reflectance interference microscopy (RICM) to measure the biomechanics
of glycocalyx components. Using these diverse methodologies, we hope
to develop a comprehensive model of the endothelial glycocalyx as it
relates to mechano-transduction and barrier function.
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Mathematical Biology Program
Department of Mathematics
University of Utah
155 South 1400 East Room 233
Salt Lake City, UT 84112
rasmusse@math.utah.edu
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