Mathematical Biology Seminar
Alex Chagovetz,
Electrical Engineering, University of Utah
Wednesday November 1, 2006
3:05pm in LCB 215
"Modeling of competitive capture of DNA on biosensors"
We present a diffusion-kinetic model of multiple DNA species
interacting with the surface of biosensors (oligonucleotide
probes). Competition for binding sites results in displacement of low
affinity species by high affinity species in the transient
regime. This mechanism helps to explain origins of specificity of
molecular recognition and defines dynamic range of specific
recognition as a function of time. Non-linear effects of transient
multi-species capture can be used for quantitation of specific targets
in the sample without using tags (tagless detection). Experimental
results confirm theoretical predictions and justify the need for
real-time signal acquisition in order to convert DNA microarrays into
the tool for quantitative analysis.
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Mathematical Biology Program
Department of Mathematics
University of Utah
155 South 1400 East Room 233
Salt Lake City, UT 84112
rasmusse@math.utah.edu
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